ABSTRACT
OBJECTIVE@#To determine the effect and molecular mechanism of DNA damage caused by suicide gene therapy system HSV-TK/GCV under Tet-On regulation in human breast cancer cell line MCF-7 infected by recombinant adeno-associated virus (rAAV).@*METHODS@#We used comet assay to detect the effect of HSV-TK/GCV suicide gene regulation system on MCF-7 DNA damage, and analyzed the expression change of relative DNA damage response active genes and proteins with RT-PCR and Western blot.@*RESULTS@#Compared with other control groups, the comet assay showed that MCF-7 cells with HSV-TK/GCV treatment had obvious comet tails, and the expression level of DNA damage response active genes and proteins changed obviously in the HSV-TK/GCV treatment group,such as ATM, p53 and p27,but CyclinE and CDK2 did not change.@*CONCLUSION@#DNA damage on MCF-7 cells is resulted from HSV-TK/GCV in suicide gene therapy system through a p53-dependent signal pathway, causing cell cycle arrest and cell death.
Subject(s)
Female , Humans , Breast Neoplasms , Genetics , Pathology , Therapeutics , DNA Damage , Dependovirus , Genetics , Ganciclovir , Metabolism , Pharmacology , Gene Expression Regulation, Neoplastic , Genes, Transgenic, Suicide , Genetics , Genetic Therapy , MCF-7 Cells , Recombinant Fusion Proteins , Genetics , Metabolism , Simplexvirus , Thymidine Kinase , GeneticsABSTRACT
Objective:To observe the curative effect of traditional Chinese medicine on non-alcoholic fatty liver patients and CTN(computerized tomographynumber)of liver,than to appraise the role of assessment and diagnosis on non-alcoholic fatty liver for CT.Methods:60 patients with non-alcoholic fatty liver were divided into treatment group and contrast group in average,the patients in treatment group were treated by Xiaozhihugan capsule and Dangfeiliganning capsule,and those in contrast group were treated by Kezhi capsule and Shuifeijibin capsule.Changes of symptom,liver function,blood fat,ultrasonic report and liver CTN were observed.Results:In the treat group,the liver function,blood fat and liver CT value were improved obviously,which had superiority to that of contrast group.CT can diagnose the degree of fatty liver accurately and appraise curative effect,but ultrasonic report has no specific data and is inconvenient to statistics.Conclusions:Treatment group has notable curative effect on non-alcoholic fatty liver;CT is the important index of diagnosis and appraisement of curative effect on non-alcoholic fatty liver.